Prune tips shorter than maxBranchCull. */
| 35 | |
| 36 | /** Prune tips shorter than maxBranchCull. */ |
| 37 | static inline |
| 38 | size_t trimSequences(SequenceCollectionHash* seqCollection, |
| 39 | unsigned maxBranchCull) |
| 40 | { |
| 41 | typedef SequenceCollectionHash Graph; |
| 42 | typedef graph_traits<Graph>::vertex_descriptor V; |
| 43 | typedef Graph::SymbolSetPair SymbolSetPair; |
| 44 | |
| 45 | Timer timer("TrimSequences"); |
| 46 | std::cout << "Pruning tips shorter than " |
| 47 | << maxBranchCull << " bp...\n"; |
| 48 | size_t numBranchesRemoved = 0; |
| 49 | |
| 50 | for (Graph::iterator iter = seqCollection->begin(); |
| 51 | iter != seqCollection->end(); ++iter) { |
| 52 | if (iter->second.deleted()) |
| 53 | continue; |
| 54 | |
| 55 | extDirection dir; |
| 56 | // dir will be set to the trimming direction if the sequence |
| 57 | // can be trimmed. |
| 58 | SeqContiguity status = checkSeqContiguity(*iter, dir); |
| 59 | |
| 60 | if (status == SC_CONTIGUOUS) |
| 61 | continue; |
| 62 | else if(status == SC_ISLAND) |
| 63 | { |
| 64 | // remove this sequence, it has no extensions |
| 65 | seqCollection->mark(iter->first); |
| 66 | numBranchesRemoved++; |
| 67 | continue; |
| 68 | } |
| 69 | |
| 70 | BranchRecord currBranch(dir); |
| 71 | V currSeq = iter->first; |
| 72 | while(currBranch.isActive()) |
| 73 | { |
| 74 | SymbolSetPair extRec; |
| 75 | int multiplicity = -1; |
| 76 | bool success = seqCollection->getSeqData( |
| 77 | currSeq, extRec, multiplicity); |
| 78 | assert(success); |
| 79 | (void)success; |
| 80 | processLinearExtensionForBranch(currBranch, |
| 81 | currSeq, extRec, multiplicity, maxBranchCull); |
| 82 | } |
| 83 | |
| 84 | // The branch has ended check it for removal, returns true if |
| 85 | // it was removed. |
| 86 | if(processTerminatedBranchTrim(seqCollection, currBranch)) |
| 87 | { |
| 88 | numBranchesRemoved++; |
| 89 | } |
| 90 | seqCollection->pumpNetwork(); |
| 91 | } |
| 92 | |
| 93 | size_t numSweeped = removeMarked(seqCollection); |
| 94 |
no test coverage detected